360 MMP FRET Substrate IV

This substrate has a fluorescent Trp residue in subsite P’2 and a dinitrophenol (Dnp) quenching group at the N-terminus. The quenching of the Trp fluorescence in the intact substrate is relieved upon hydrolysis of the P1-P’1 bond, giving rise to a continuously recording fluorescence assay. The residues placed in subsites P3-P’1 and P’3 is optimized for MMP-3, while Arg is placed in P’4 to enhance solubility. The substrate hydrolysis rate rivals or exceeds those of the corresponding natural protein substrates or other synthetic peptides. The solubility of this substrate in assay buffer exceeds the Km value for each reaction, allowing accurate determination of the kinetic parameters. Abs/Em = 280/360 nm.

Specifications

Product Name:

360 MMP FRET Substrate IV

Synonyms:      

Dnp-PYAYWMR

Sequence (one-letter code)

Dnp-PYAYWMR

Sequence (three-letter code)

Dnp - Pro - Tyr - Ala - Tyr - Trp - Met - Arg - OH

Molecular Weight:

1152.3

Excitation (nm):

280

Emission (nm):

360

Solubility:

DMSO

Purity (HPLC):

≥ 95%

Shipping Conditions:

Room temperature

Storage Conditions:

Store at -20 ºC and protect from light.

Regulatory Statement:

For Research Use Only. 

360 MMP FRET Substrate IV

  • $209.00
  • Ex Tax: $209.00

Available Options

Tags: 360, mmp, fret, substrate, fluorescent, labeled, peptides