6-FAM azide

6-FAM azide

6-FAM azide is an excellent alkyne-reactive fluorescent reagent for labeling azide-containing biological molecules through click chemistry.  Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moities to label and detect a molecule of interest using a two-step procedure. The two-step reaction procedure involves a copper-catalyzed triazole formation of an azide and an alkyne. Click reactions have several characteristics: the reaction between the detection moieties is efficient; no extreme temperatures or solvents are required; the reaction product is stable; the components of the reaction are bioinert; and perhaps most importantly, no side reactions occur – the label and detection tags react selectively and specifically with one another. Unlike traditional chemical reactions utilizing succinimidyl esters or maleimides that target amines and sulfhydryls – functional groups that are not unique – click chemistry-labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background.

Specifications

Product Name:      

6-FAM-azide

Synonyms:      

6-Carboxyfluorescein-azide; N-(3-azidopropyl)-3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxamide 

CAS Number:

N/A

Molecular Weight:

458.42

Molecular Formula:

C24H18N4O6

Excitation (nm):

495

Emission (nm):

520

Solubility:

DMSO, DMF

Purity (HPLC):

≥ 95%

Shipping Conditions:

Room temperature

Storage Conditions:

Store at -20 ºC and protect from light.

Regulatory Statement:

For Research Use Only. 

  • $109.00
  • Ex Tax: $109.00

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Tags: 6-fam, azide, fluorescent, azides