• 5-FAM alkyne

5-FAM alkyne is an excellent azide-reactive fluorescent reagent for labeling alkyne-containing biological molecules through click chemitry.  Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moities to label and detect a molecule of interest using a two-step procedure. The two-step reaction procedure involves a copper-catalyzed triazole formation of an azide and an alkyne. Click reactions have several characteristics: the reaction between the detection moieties is efficient; no extreme temperatures or solvents are required; the reaction product is stable; the components of the reaction are bioinert; and perhaps most importantly, no side reactions occur – the label and detection tags react selectively and specifically with one another. Unlike traditional chemical reactions utilizing succinimidyl esters or maleimides that target amines and sulfhydryls – functional groups that are not unique – click chemistry-labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background.

Specifications

Product Name:      

5-FAM Alkyne

Synonyms:      

5- carboxyfluorescein, propargylamide

CAS Number:

510758-19-7

Molecular Weight:

413.38

Molecular Formula:

C25H15O6N

Excitation (nm):

494

Emission (nm):

521

Solubility:

DMSO, DMF

Purity (HPLC):

≥ 95%

Shipping Conditions:

Room temperature

Storage Conditions:

Freeze at -20 °C, desiccate, and protect from light.

Regulatory Statement:

For Research Use Only. 

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5-FAM alkyne

  • $109.00

  • Ex Tax: $109.00

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Tags: 5-fam, alkyne, fluorescent, alkynes