5(6)-FAM alkyne is an excellent azide-reactive fluorescent reagent for labeling alkyne-containing biological molecules through click chemitry. Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moities to label and detect a molecule of interest using a two-step procedure. The two-step reaction procedure involves a copper-catalyzed triazole formation of an azide and an alkyne. Click reactions have several characteristics: the reaction between the detection moieties is efficient; no extreme temperatures or solvents are required; the reaction product is stable; the components of the reaction are bioinert; and perhaps most importantly, no side reactions occur – the label and detection tags react selectively and specifically with one another. Unlike traditional chemical reactions utilizing succinimidyl esters or maleimides that target amines and sulfhydryls – functional groups that are not unique – click chemistry-labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background.
Specifications
Product Name: |
5(6)-FAM alkyne |
Synonyms: |
5- (and -6) – carboxyfluorescein, propargylamide |
CAS Number: |
N/A |
Molecular Weight: |
413.38 |
Molecular Formula: |
C24H15NO6 |
Excitation (nm): |
494 |
Emission (nm): |
521 |
Solubility: |
DMSO, DMF |
Purity (HPLC): |
≥ 95% |
Shipping Conditions: |
Room temperature |
Storage Conditions: |
Store at -20 ºC and protect from light. |
Regulatory Statement: |
For Research Use Only. |