5(6)-FAM, SE

5(6)-FAM, SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5. The amide linkage from the coupling reaction is much more stable than the thiourea linkage formed from the coupling of an amine and an isothiocyanate such as FITC.

Specifications

Product Name:      

5(6)-FAM, SE

Synonyms:      

5 - (and - 6) - Carboxyfluorescein, succinimidyl ester

CAS Number:

117548-22-8

Molecular Weight:

473.39

Molecular Formula:

C25H15NO9

Excitation (nm):

494

Emission (nm):

519

Solubility:

DMSO, DMF

Purity (HPLC):

≥ 95%

Shipping Conditions:

Room temperature

Storage Conditions:

Store in freezer (-5 to -30°C) and protect from light.

Regulatory Statement:

For Research Use Only. 

5(6)-FAM, SE

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Tags: 5(6)-fam, nhs, ester, dyes