5-(and-6)-carboxyl-2’,7’-dichlorofluorescein diacetate

5-(and-6)-carboxyl-2’,7’-dichlorofluorescein diacetate

5-(and-6)-carboxyl-2’,7’-dichlorofluorescein diacetate (Carboxy-DCFDA, CDCF) can passively diffuse into cells and is colorless and nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the fluorescent fluorophore, 5-(and-6)-carboxy-2',7' -dichlorofluorescein. With a pKa of 4.8, this dye can be used as an acidic pH sensor.  It has been used as a fluorescent substrate/probe to study the distribution and mechanism of action of multidrug resistance-associated protein 2 (Mrp2).

Specifications

Product Name:      

5-(and-6)-carboxyl-2’,7’-dichlorofluorescein diacetate

Synonyms:      

Carboxy-DCFDA, Spiro[isobenzofuran-1(3H),9'-(9H)xanthene)-ar-carboxylic acid, 3',6'-bis(acetyloxy)-2',7'-dichloro-3-oxo

CAS Number:

127770-45-0

Molecular Weight:

529.28

Molecular Formula:

C25H14Cl2O9

Excitation (nm):

470

Emission (nm):

529

Solubility:

DMSO, DMF

Purity (HPLC):

≥ 95%

Shipping Conditions:

Room temperature

Storage Conditions:

Store Store in freezer (-5 to -30°C) and protect from light.

Regulatory Statement:

For Research Use Only. 

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Tags: 5-(and-6)-carboxyl-2’7’-dichlorofluorescein, diacetate, carboxylic, acid, dyes