5(6)-FAM

5(6)-FAM

Mixed isomer of 5 and 6-FAM.  Fluorescein is one of the most popular green fluorescent reagents used for in situ labeling of peptides, proteins and nucleotides.  It reacts with primary amines via carbodiimide activation of the carboxylic acid.  Fluorescein has relatively high absorptivity, excellent fluorescence quantum yield, good water solubility, and an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.  The cell-impermeant 5,6-FAM can also be used as a nonfixable polar tracer to investigate fusion, lysis and gap-junctional communication and to detect changes in cell or liposome volume.  It has also been used to prepare various small fluorescent molecules.

Specifications

Product Name:      

5(6)-FAM

Synonyms:      

5 - (and - 6) - Carboxyfluorescein

CAS Number:

72088-94-9

Molecular Weight:

376.32

Molecular Formula:

C21H12O7

Excitation (nm):

494

Emission (nm):

519

Solubility:

DMSO, DMF

Purity (HPLC):

≥ 95%

Shipping Conditions:

Room temperature

Storage Conditions:

Store at room temperature and protect from light.

Regulatory Statement:

For Research Use Only. 

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Tags: 5(6)-fam, carboxylic, acid, dyes