HaloTag is a self-labeling protein derived from the haloalkane dehalogenase DhaA from Rhodococcus rhodochrous. Its active site reacts with chloroalkane substrates to form an irreversible bond. The covalent linkage between the HaloTag ligand and HaloTag protein enables rapid isolation and purification. HaloTag diAcFAM Ligand is a cell permeable HaloTag fluorescent ligand and can be used to label intracellular proteins (494Ex/526Em after hydrolysis). It is an equivalent of Promega product G8272 and G8273.
| Product Name | HaloTag diAcFAM Ligand |
| Synonyms | diAcFAM-Halo |
| CAS Number | |
| Related CAS | |
| Molecular Formula | C35H36ClNO10 |
| Molecular Weight | 666.12 |
| Purity (HPLC) | >= 95% |
| Physical Appearance | Off white Solid |
| Optical Properties | Abs/Em Maxima: 494/526 nm |
| Solubility | DMSO, DMF |
| Shipping Conditions | Shipped on gel packs |
| Storage Conditions | Store at -20°C and protected from light |
| Shelf Life | 12 months after the date of delivery |
| Regulatory Statement | For Research Use Only |
References
Wilhelm, J., Kuhn, S., Tarnawski, M., Gotthard, G., Tunnermann, J., Tanzer, T., Karpenko, J., Mertes, N., Xue, L., Uhrig, U., Reinstein, J., Hiblot, J., & Johnsson, K. (2021). Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag. Biochemistry, 60(33), 2560–2575. https://doi.org/10.1021/acs.biochem.1c00259
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