Hoechst-Cy5 is a small-molecule DNA-specific fluorophore. It can be used as a fast, simple, and robust DNA stain for high-quality superresolution chromatin imaging on clinically processed tissue samples. In a comparative study of STORM(Stochastic optical reconstruction microscopy) imaging of genomic DNA using tissue section of mouse intestinal tissue prepared with standard clinical protocol, the images from Hoechst-Cy5 STORM showed the best-resolved DNA nanodomains when compared with TOTO-3 Iodide (TOTO-3), NucSpot Live 650 (Live-650), and Hoechst Janelia Fluor 646 (Hoechst-JF646). Hoechst-Cy5 significantly facilitates routine examination of superresolved chromatin structure from various pathological conditions directly on clinically processed tissue. Excitation maximum = 650 nm; emission maximum = 660 nm.
| Product Name | Hoechst-Cy5 |
| Synonyms | |
| CAS Number | N/A |
| Related CAS | |
| Molecular Formula | C67H81N10O5 |
| Molecular Weight | 1106.449 |
| Purity (HPLC) | >= 95% |
| Physical Appearance | Dark Blue Solid |
| Optical Properties | Abs/Em Maxima: 650/660nm |
| Solubility | DMSO, DMF |
| Shipping Conditions | Shipped on gel packs |
| Storage Conditions | Store at -20°C and protected from light |
| Shelf Life | 12 months after the date of delivery |
| Regulatory Statement | For Research Use Only |
References
Xu, J., Sun, X., Kim, K., Brand, R. M., Hartman, D., Ma, H., Brand, R. E., Bai, M., & Liu, Y. (2022). Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe. In Sci. Adv (Vol. 8). https://www.science.org DOI: 10.1126/sciadv.abm8293
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